期刊
JOURNAL OF NANOBIOTECHNOLOGY
卷 20, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12951-022-01312-0
关键词
Thermal stability; Vascular imaging; Quantum dots; NIR-IIb window; Intraoperative imaging navigation
资金
- National Key R&D Program of China [2016YFC1100300]
- National Natural Science Foundation of China [81772339, 81972129, 81911530136, 81811530750, 82072521]
- Key Clinical Medicine Center of Shanghai [2017ZZ01006]
- Sanming Project of Medicine in Shenzhen [SZSM201612078]
- Shanghai Rising-Star Project [18QB1400500]
- The Introduction Project of Clinical Medicine Expert Team for Suzhou [SZYJTD201714]
- Development Project of Shanghai Peak Disciplines-Integrative Medicine [20180101]
- Shanghai Talent Development Funding Scheme [2020080]
- Shanghai Sailing Program [21YF1404100]
- Interdisciplinary Program of Shanghai Jiao Tong University [YG2019QNB09]
- Shanghai Municipal Key Clinical Specialty [shslczdzk00901]
- Natural Science Foundation of Shanghai Committee of Science and Technology [21ZR1446000]
This study introduces a novel hybrid PbS/ZnS QDs nanostructure for stable fluorescence signals in long-time intraoperative imaging navigation. Compared to NIR-Ila fluorescence imaging, NIR-IIb vascular fluorescence imaging achieved larger penetration depth, higher signal/background ratios and nearly zero endogenous tissue autofluorescence.
Surgeons face great challenges in acquiring high-performance imaging because fluorescence probes with desired thermal stability remains rare. Here, hybrid lead sulfide/zinc sulfide quantum dots (PbS/ZnS QDs) nanostructures emitting in the long-wavelength end of the second near-infrared (NIR-11b) window were synthesized and conjugated with Ribonuclease-A (RNase A). Such formed RNase A@PbS/ZnS QDs exhibited strong NIR Ilb fluorescence and thermal stability, as supported by the photoluminescent emission assessment at different temperatures. This will allow the RNase A@PbS/ZnS QDs to provide stable fluorescence signals for long-time intraoperative imaging navigation, despite often happened, undesirable thermal accumulation in vivo. Compared to NIR-Ila fluorescence imaging, NIR-Ilb vascular fluorescence imaging achieved larger penetration depth, higher signal/background ratios and nearly zero endogenous tissue autofluorescence. Moreover, these QDs illustrate the reliability during the real-time and long-time precise assessment of flap perfusion by clearly visualizing microvasculature map. These findings contribute to intraoperative imaging navigation with higher precision and lower risk.
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