Microparticles: biogenesis, characteristics and intervention therapy for cancers in preclinical and clinical research

Extracellular vesicles (EVs), spherical biological vesicles, mainly contain nucleic acids, proteins, lipids and metabolites for biological information transfer between cells. Microparticles (MPs), a subtype of EVs, directly emerge from plasma membranes, and have gained interest in recent years. Specific cell stimulation conditions, such as ultraviolet and X-rays irradiation, can induce the release of MPs, which are endowed with unique antitumor functionalities, either for therapeutic vaccines or as direct antitumor agents. Moreover, the size of MPs (100-1000 nm) and their spherical structures surrounded by a lipid bilayer membrane allow MPs to function as delivery vectors for bioactive antitumor compounds, with favorable phamacokinetic behavior, immunostimulatory activity and biological function, without inherent carrier-specific toxic side effects. In this review, the mechanisms underlying MP biogenesis, factors that influence MP production, properties of MP membranes, size, composition and isolation methods of MPs are discussed. Additionally, the applications and mechanisms of action of MPs, as well as the main hurdles for their applications in cancer management, are introduced.

Automated summary

This article reviews the subtype of extracellular vesicles (EVs) called microparticles (MPs), which function as delivery vectors for bioactive antitumor compounds. MPs, with their unique antitumor functionalities, have gained interest for their potential use in therapeutic vaccines and direct antitumor agents. The mechanisms underlying MP biogenesis, factors that influence MP production, properties of MP membranes, size, composition, and isolation methods are discussed. Additionally, the applications, mechanisms of action, and main hurdles for MP use in cancer management are introduced.