期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 291, 期 22, 页码 11619-11634出版社
ELSEVIER
DOI: 10.1074/jbc.M115.713370
关键词
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资金
- Japan Society for the Promotion of Science (JSPS) [KAKENHI 20570188]
- Grants-in-Aid for Scientific Research [15K07161] Funding Source: KAKEN
The human transcription factor DNA replication-related element-binding factor (hDREF) is essential for the transcription of a number of housekeeping genes. The mechanisms underlying constitutively active transcription by hDREF were unclear. Here, we provide evidence that hDREF possesses small ubiquitin-like modifier (SUMO) ligase activity and can specifically SUMOylate Mi2 alpha, an ATP-dependent DNA helicase in the nucleosome remodeling and deacetylation complex. Moreover, immunofluorescent staining and biochemical analyses showed that coexpression of hDREF and SUMO-1 resulted in dissociation of Mi2 alpha from chromatin, whereas a SUMOylation-defective Mi2 alpha mutant remained tightly bound to chromatin. Chromatin immunoprecipitation and quantitative RT-PCR analysis demonstrated that Mi2 alpha expression diminished transcription of the ribosomal protein genes, which are positively regulated by hDREF. In contrast, coexpression of hDREF and SUMO-1 suppressed the transcriptional repression by Mi2 alpha. These data indicate that hDREF might incite transcriptional activation by SUMOylating Mi2 alpha, resulting in the dissociation of Mi2 alpha from the gene loci. We propose a novel mechanism for maintaining constitutively active states of a number of hDREF target genes through SUMOylation.
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