期刊
FRONTIERS IN MICROBIOLOGY
卷 13, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2022.880248
关键词
Klebsiella michiganensis; IncX3-bla(NDM-5) plasmid; fitness cost; stability; KoI
类别
资金
- Japan Agency for Medical Research and Development (AMED) [JP20wm0125007]
This study highlights the emergence of a high-risk Klebsiella michiganensis clone harboring carbapenemase genes. It confirms that the recent spread of IncX3-bla(NDM-5) plasmids might be due to their low fitness cost and stability, but not their competitive prowess.
Klebsiella michiganensis is emerging as an important human pathogen of concern especially strains with plasmid-mediated carbapenemase genes. The IncX3-bla(NDM-5) plasmid has been described as the primary vector for bla(NDM-5) dissemination. However, whether strains with this plasmid have any competitive edge remain largely unexplored. We characterized a bla(NDM-5)-producing Klebsiella michiganensis strain (KO_408) from Japan and sought to understand the driving force behind the recent dissemination of IncX3-bla(NDM-5) plasmids in different bacterial hosts. Antibiotic susceptibility testing, conjugation, and whole-genome sequencing were performed for KO_408, a clinical isolate recovered from a respiratory culture. Fitness, stability, and competitive assays were performed using the IncX3-bla(NDM-5) plasmid, pKO_4-NDM-5. KO_408 was ascribed to a novel sequence type, ST256, and harbored resistance genes conforming to its MDR phenotype. The bla(NDM-5) gene was localized on the ~44.9 kb IncX3 plasmid (pKO_4-NDM-5), which was transferable in the conjugal assay. The acquisition of pKO_4-NDM-5 did not impose any fitness burden and showed high stability in the host cells. However, transformants with pKO_4-NDM-5 were outcompeted by their host cells and transconjugants with the IncX3-bla(OXA-181) plasmid. The genetic environment of bla(NDM-5) in pKO_4-NDM-5 has been previously described. pKO_4-NDM-5 showed a close phylogenetic distance with seven similar plasmids from China. KO_408 clustered with strains within the KoI phylogroup, which is closely associated with carbapenemase genes. This study highlights the emergence of a high-risk Klebsiella michiganensis clone harboring carbapenemase genes and affirms that the recent spread of IncX3-bla(NDM-5) plasmids might be due to their low fitness cost and stability but not their competitive prowess.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据