LuxS in Lactobacillus plantarum SS-128 Improves the Texture of Refrigerated Litopenaeus vannamei: Mechanism Exploration Using a Proteomics Approach

This study illustrated the texture changes of Shewanella baltica-inoculated Litopenaeus vannamei during refrigerated storage with the exogenous addition of Lactobacillus plantarum SS-128. The group inoculated with SS-128 had an improved texture compared with that inoculated with the luxS-mutant group (Delta luxS). Proteomics were conducted to analyze the protein alterations in L. vannamei and supernatant, respectively. During storage, many texture-related proteins, including myosin heavy chain and beta-actin, were maintained due to luxS. Some endogenous enzymes related to the energy metabolism and hydrolysis of L. vannamei were downregulated. The luxS-induced interaction with S. baltica showed significant changes in the expression of some critical enzymes and pathways. The ATP-dependent zinc metalloprotease FtsH and protease subunit HslV were downregulated, and the oxidative phosphorylation and glycosaminoglycan degradation pathways in S. baltica were inhibited, resulting in the slow deterioration of L. vannamei. By exploring the mechanism underlying SS-128-led manipulation of the metabolism of spoilage bacteria, we clarified the texture maintenance mechanism of luxS in SS-128, providing theoretical evidence for SS-128 application in food preservation.

Automated summary

This study investigated the texture changes of Shewanella baltica-inoculated Litopenaeus vannamei during refrigerated storage and the impact of exogenously added Lactobacillus plantarum SS-128 on these changes. Results showed that SS-128 improved the texture of L. vannamei compared to the luxS-mutant group. Proteomics analysis revealed protein alterations in L. vannamei and the supernatant. The study also demonstrated that luxS played a role in maintaining texture-related proteins and regulating the expression of enzymes involved in energy metabolism and hydrolysis in L. vannamei. The interaction between luxS and S. baltica influenced the expression of critical enzymes and pathways, leading to the deterioration of L. vannamei.