4.7 Review

The Viable But Non-Culturable State of Listeria monocytogenes in the One-Health Continuum

出版社

FRONTIERS MEDIA SA
DOI: 10.3389/fcimb.2022.849915

关键词

zoonosis; VBNC; dormancy; asymptomatic infections; foodborne pathogen; risk assessment; pregnancy; infectious diseases

资金

  1. ANR PERMALI [ANR20-CE35-0001-01]
  2. iXcore Foundation
  3. INRAE-MICA division (AAP 2019-2020)

向作者/读者索取更多资源

The VBNC state of bacteria, particularly in the case of Lm pathogens, has important implications for environmental, agricultural, and food industry settings, as well as potential risks in the host. The hypothesis that VBNC forms contribute to adaptation, persistence, and transmission of Lm between different ecological niches is put forth.
Many bacterial species, including several pathogens, can enter a so-called viable but non-culturable (VBNC) state when subjected to stress. Bacteria in the VBNC state are metabolically active but have lost their ability to grow on standard culture media, which compromises their detection by conventional techniques based on bacterial division. Under certain conditions, VBNC bacteria can regain their growth capacity and, for pathogens, their virulence potential, through a process called resuscitation. Here, we review the current state of knowledge of the VBNC state of Listeria monocytogenes (Lm), a Gram-positive pathogenic bacterium responsible for listeriosis, one of the most dangerous foodborne zoonosis. After a brief summary of characteristics of VBNC bacteria, we highlight work on VBNC Lm in the environment and in agricultural and food industry settings, with particular emphasis on the impact of antimicrobial treatments. We subsequently discuss recent data suggesting that Lm can enter the VBNC state in the host, raising the possibility that VBNC forms contribute to the asymptomatic carriage of this pathogen in wildlife, livestock and even humans. We also consider the resuscitation and virulence potential of VBNC Lm and the danger posed by these bacteria to at-risk individuals, particularly pregnant women. Overall, we put forth the hypothesis that VBNC forms contribute to adaptation, persistence, and transmission of Lm between different ecological niches in the One-Health continuum, and suggest that screening for healthy carriers, using alternative techniques to culture-based enrichment methods, should better prevent listeriosis risks.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据