Ozone-derived Oxysterols Affect Liver X Receptor (LXR) Signaling A POTENTIAL ROLE FOR LIPID-PROTEIN ADDUCTS

When inhaled, ozone (O-3) interacts with cholesterols of airway epithelial cell membranes or the lung-lining fluid, generating chemically reactive oxysterols. The mechanism by which O-3-derived oxysterols affect molecular function is unknown. Our data show that in vitro exposure of human bronchial epithelial cells to O-3 results in the formation of oxysterols, epoxy-cholesterol-alpha and -beta and secosterol A and B (Seco A and Seco B), in cell lysates and apical washes. Similarly, bronchoalveolar lavage fluid obtained from human volunteers exposed to O-3 contained elevated levels of these oxysterol species. As expected, O-3-derived oxysterols have a pro-inflammatory effect and increase NF-kappa B activity. Interestingly, expression of the cholesterol efflux pump ATP-binding cassette transporter 1 (ABCA1), which is regulated by activation of the liver X receptor (LXR), was suppressed in epithelial cells exposed to O-3. Additionally, exposure of LXR knock-out mice to O-3 enhanced pro-inflammatory cytokine production in the lung, suggesting LXR inhibits O-3-induced inflammation. Using alkynyl surrogates of O-3-derived oxysterols, our data demonstrate adduction of LXR with Seco A. Similarly, supplementation of epithelial cells with alkynyl-tagged cholesterol followed by O-3 exposure causes observable lipid-LXR adduct formation. Experiments using Seco A and the LXR agonist T0901317 (T09) showed reduced expression of ABCA1 as compared with stimulation with T0901317 alone, indicating that Seco A-LXR protein adduct formation inhibits LXR activation by traditional agonists. Overall, these data demonstrate that O-3-derived oxysterols have pro-inflammatory functions and form lipid-protein adducts with LXR, thus leading to suppressed cholesterol regulatory gene expression and providing a biochemical mechanism mediating O-3-derived formation of oxidized lipids in the airways and subsequent adverse health effects.