期刊
CLINICAL EPIGENETICS
卷 14, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13148-022-01257-z
关键词
Epigenetics; Metabolites; Lipids; Multi-omics; Obesity; Lipodystrophy; Bariatric surgery; Classification; Innate immune cells; Cardiometabolic syndrome
资金
- British Heart Foundation Cambridge Centre of Excellence
- MRC Clinical Research Training Fellowships [MR/R002363/1]
- Wellcome Trust [WT 107064]
- MRC Metabolic Disease Unit [MRC_MC_UU_12012.1]
- National Institute for Health Research (NIHR) Cambridge Biomedical Research Centre
- NIHR Rare Disease Translational Research Collaboration
- NHS Health Education England
- Medical Research Council [MC_UU_00002/13]
- NIHR Cambridge Biomedical Research Centre
- British Heart Foundation [FS/18/53/33863]
- Isaac Newton fellowship
- NIHR Leicester Biomedical Research Centre
- John and Lucille Van Geest Foundation
This study aims to improve the understanding of cardiometabolic syndrome pathophysiology and its relationship with thrombosis by generating a multi-omic disease signature. The results showed that extreme phenotype groups could be distinguished from lean individuals and from each other across all data layers. In addition, the gene expression landscape of the obese group changed after surgical intervention, indicating improvement in specific pathways.
Background This work is aimed at improving the understanding of cardiometabolic syndrome pathophysiology and its relationship with thrombosis by generating a multi-omic disease signature. Methods/results We combined classic plasma biochemistry and plasma biomarkers with the transcriptional and epigenetic characterisation of cell types involved in thrombosis, obtained from two extreme phenotype groups (morbidly obese and lipodystrophy) and lean individuals to identify the molecular mechanisms at play, highlighting patterns of abnormal activation in innate immune phagocytic cells. Our analyses showed that extreme phenotype groups could be distinguished from lean individuals, and from each other, across all data layers. The characterisation of the same obese group, 6 months after bariatric surgery, revealed the loss of the abnormal activation of innate immune cells previously observed. However, rather than reverting to the gene expression landscape of lean individuals, this occurred via the establishment of novel gene expression landscapes. NETosis and its control mechanisms emerge amongst the pathways that show an improvement after surgical intervention. Conclusions We showed that the morbidly obese and lipodystrophy groups, despite some differences, shared a common cardiometabolic syndrome signature. We also showed that this could be used to discriminate, amongst the normal population, those individuals with a higher likelihood of presenting with the disease, even when not displaying the classic features.
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