期刊
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
卷 65, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.algal.2022.102737
关键词
Adenosine-5'-monophosphate deaminase; Neopyropia yezoensis; Umami substance; Genetic transformation
资金
- National Key Research and Development Program of China [2018YFD0901500]
- National Natural Science Foundation of China [41906105, 41876163]
- Research Fund for the Taishan Scholar Project of Shandong Province [tspd20210316]
- Key Deployment Project of the Centre for Ocean Mega-Research of Science [COMS2019Q02]
- Major Scientific and Technological Innovation Projects in Shandong Province [2019JZZY010815]
- China Agriculture Research System of MOF and MARA [CARS-50]
- Qingdao Postdoctoral Funding [Y9KY161]
This study utilized precision molecular breeding technology to improve the quality of Neopyropia yezoensis. The overexpression mutants showed higher IMP content and growth rate, while knockdown mutants exhibited genetic compensation. These findings provide important insights for further genetic transformation research.
Precision molecular breeding technology plays an important role in improving the yield and quality of cultivated marine crops. In this study, we aimed to improve the quality of Neopyropia yezoensis, one of the important economic seaweeds. We constructed overexpression (OE)-, co-suppression (Ci)-, and RNAi (Ri)-type mutants of Nyampd (AMP deaminase of N. yezoensis), which could catalyze the decomposition of AMP into the umami substance inosine 5'- mononucleotide (IMP) through the genetic transformation system of N. yezoensis. Our results showed that the IMP content and growth rate of OE strain were significantly higher than those of wild type. In addition, in knockdown strains (Ci/Ri), a phenomenon similar to genetic compensation in knockout research occurred, i.e., the expression of Nyampd2 (isozyme of Nyampd) was significantly upregulated in Ri/Ci strains, and the IMP content increased in Ci/Ri strains. These results provide important knowledge for precise molecular breeding and genetic transformation research of N. yezoensis.
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