期刊
TOXINS
卷 14, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/toxins14040239
关键词
T-2 toxin; G; candidum; phenyllactic acid; F; sporotrichioides; F; langsethiae; biocontrol agent; mycotoxin; barley; micro-malting
资金
- Agence Nationale de la Recherche [ANR-16-CE21-0011]
This study evaluated the effects of inoculum concentration and inoculation method of Geotrichum candidum (G. candidum) on the concentrations of phenyllactic acid (PLA) and T-2 toxin. The results showed that inoculating G. candidum at a concentration of 0.4 g/L in freeze-dried form led to PLA production and a significant reduction in T-2 toxin concentration. Additionally, using the activated form of G. candidum at the same concentration further enhanced PLA concentration and improved T-2 toxin reduction. Similar results were obtained in experiments conducted on artificially infected barley kernels mimicking the malting step.
In malt production, steeping and germination steps offer favorable environmental conditions for fungal proliferation when barley is already contaminated by Fusarium species, T-2 toxin producers. However, the use of G. candidum as a biocontrol agent can prevent this proliferation. Indeed, in previous work, a correlation between phenyllactic acid (PLA) production by G. candidum and the reduction in Fusarium sporotrichioides and F. langsethiae growth and T-2 toxin concentration was demonstrated. In the present study, to improve the efficiency of G. candidum, the effects of the inoculum concentration and the inoculation method of G. candidum on PLA and T-2 toxin concentrations were evaluated. First, co-culture experiments with Fusarium species and G. candidum were conducted in a liquid synthetic medium. The results showed that inoculation of G. candidum in the freeze-dried form at 0.4 g/L allowed the production of PLA from the second day of incubation associated with a reduction in T-2 toxin concentration of 82% and 69% produced by F. sporotrichioides and F. langsethiae, respectively. Moreover, the activated form of G. candidum at 0.4 g/L enhanced PLA concentration leading to better T-2 toxin reduction. Second, experiments were conducted on artificially infected barley kernels with both Fusarium species under conditions mimicking the malting step. As for co-culture experiments, the use of the activated form of G. candidum was established as the best condition for T-2 toxin concentration reduction for a 3 day malting period.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据