期刊
POLYMERS
卷 14, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/polym14112176
关键词
quaternized chitosan; polyplex; nanoparticles; interleukin-12; gene delivery
资金
- Shiraz University of Medical Sciences [89-0136-2456, 26050]
- Slovak Grant Agency VEGA [2/0140/20]
- Slovak Research and Development Agency [APVV-18-0480, APVV-20-0202]
This study evaluated the transfection efficiency and cytotoxicity of N-[(2-hydroxy-3-trimethylammonium)propyl] chitosan salt (HTCS) as a gene carrier. HTCS was synthesized and used for the delivery of the interleukin-12 (IL-12) gene plasmid. The results showed that HTCS could effectively condense the IL-12 plasmid into polyplexes and demonstrated higher hIL-12 production compared to CS and polyethylenimine polyplexes in cell transfection experiments.
Cationic polysaccharides are capable of forming polyplexes with nucleic acids and are considered promising polymeric gene carriers. The objective of this study was to evaluate the transfection efficiency and cytotoxicity of N-[(2-hydroxy-3-trimethylammonium)propyl] chitosan salt (HTCS), a quaternary ammonium derivative of chitosan (CS), which benefits from non-ionizable positive charges. In this work, HTCS with a full quaternization of amino groups and a molar mass of 130,000 g center dot mol(-1) was synthesized to use for delivery of a plasmid encoding the interleukin-12 (IL-12) gene. Thus, a polyplex based on HTCS and the IL-12 plasmid was prepared and then was characterized in terms of particle size, zeta potential, plasmid condensation ability, and protection of the plasmid against enzymatic degradation. We showed that HTCS was able to condense the IL-12 plasmid by the formation of polyplexes in the range of 74.5 +/- 0.75 nm. The level of hIL-12 production following the transfection of the cells with HTCS polyplexes at a C/P ratio of 8:1 was around 4.8- and 2.2-fold higher than with CS and polyethylenimine polyplexes, respectively. These findings highlight the role of HTCS in the formation of polyplexes for the efficient delivery of plasmid DNA.
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