期刊
POLYMERS
卷 14, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/polym14071336
关键词
wheat straw; monosaccharides; xylo-oligosaccharides; hydrothermal pretreatment; enzymatic hydrolysis; downstream process; food application
资金
- MCI-UEFISCDI [TE 184, PN-III-P1-1.1-TE-2019-1748]
- National Research Development Projects to finance excellence (PFE) by the Romanian Ministry of Research and Innovation [14/2022-2024]
Xylo-oligosaccharides and fermentable sugars were extracted from wheat straw using an integrated approach. The combination of alkaline and enzymatic treatment with xylanase showed the highest concentration of fermentable sugars, while slightly reducing the yield of xylo-oligosaccharides. Refining experiments resulted in a purified fraction, but further optimization is needed to increase the yield.
Xylo-oligosaccharides are sugar oligomers with 2-7 xylose units considered non-digestible fibers that can be produced from biodegradable and low-cost biomass like wheat straw. An integrated approach consisting of hydrothermal pretreatment, alkaline treatment, enzymatic treatment and the combinations thereof was applied to overcome the recalcitrance structure of the wheat straw and allow selective fractioning into fermentable sugars and xylo-oligosaccharides. The hydrolysates and processed solids were chemically characterized by High-performance liquid chromatography and Ion chromatography, and the results were expressed as function of the severity factor and statistically interpreted. The concentration of fermentable sugars (glucose, xylose, arabinose) was the highest after the combination of alkaline and enzymatic treatment with xylanase (18 g/L sugars), while xylo-oligosaccharides (xylotriose and xylotetraose) were released in lower amounts (1.33 g/L) after the same treatment. Refining experiments were carried out to obtain a purified fraction by using anion and cation exchange chromatography. The polymer adsorber resin MN-502 showed efficient removal of salts, phenols and furan derivatives. However, the xylo-oligosaccharides yields were also slightly reduced. Although still requiring further optimization of the treatments to obtain higher purified oligomer yields, the results provide information on the production of xylo-oligosaccharides and fermentable sugars from wheat straw for potential use in food applications.
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