4.6 Article

The GhMAX2 gene regulates plant growth and fiber development in cotton

期刊

JOURNAL OF INTEGRATIVE AGRICULTURE
卷 21, 期 6, 页码 1563-1575

出版社

ELSEVIER SCI LTD
DOI: 10.1016/S2095-3119(21)63603-1

关键词

strigolactones; MAX2; cotton; hormone signaling; fiber

资金

  1. National Natural Science Foundation of China [32000146, 31470295]
  2. Natural Science Basic Research Program of Shaanxi, China [2018 JZ 3 0 0 6, 2019 JQ-0 6 2, 2020 JQ-410]
  3. Fundamental Research Funds for Central Universities, China [GK201901004, GK202 103063, GK202002005]
  4. China Postdoctoral Science Foundation [2020T130394]
  5. Young Elite Scientists Sponsorship Program by China Association for Science and Technology [2019-2021QNRC00 1]
  6. State Key Laboratory of Cotton Biology Open Fund, China [CB2020A12]

向作者/读者索取更多资源

In this study, the SLs signaling gene GhMAX2 was identified and its function in plant growth and architecture in cotton was demonstrated. Bioinformatics analysis and subcellular localization revealed that GhMAX2 was highly expressed in cotton and targeted to the nucleus. Knockdown expression of GhMAX2 resulted in significantly reduced plant height, slow growth, short internodes, and reduced fiber length in cotton. These findings suggest that GhMAX2 contributes to plant growth, architecture, and fiber elongation in cotton.
Strigolactones (SLs) are a new type of plant endogenous hormones that have been found to regulate plant growth and architecture. At present, some genes related to the biosynthesis and signaling pathway of SLs have been isolated in plants such as Arabidopsis thaliana, Pisum sativum and Oryza sativa. However, the signaling pathway and specific mechanism of SLs in cotton remain unclear. In this study, we identified the SLs signaling gene GhMAX2 and demonstrated its function in plant growth and architecture in Gossypium hirsutum. Bioinformatics analysis showed that GhMAX2 mainly consists of an alpha-helix and a random coil and includes a large number of leucine-rich repeats. GhMAX2 was highly expressed in root, stem, flower, and fibers at 20 days post-ant hesis (DPA). GhMAX2 promoter-driven beta-glucuronidase expression was present exclusively in the root, main inflorescence, flower, and silique. Subcellular localization showed that GhMAX2 is targeted to the nucleus. Heterologously expressed GhMAX2 can rescue the phenotype of Arabidopsis max2-1 mutant, indicating that the function of MAX2 is highly conserved between G. hirsutum and A. thaliana species. In addition, the knockdown expression of GhMAX2 in cotton resulted in significantly reduced plant height, slow growth, short internodes, and reduced fiber length. These findings indicate that GhMAX2 probably contributes to plant growth, architecture and fiber elongation in cotton. The study reveals insights into the roles of GhMAX2-mediated SL/KAR signaling in cotton and provides a valuable foundation for the cultivation of cotton plants in the future.

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