期刊
JOURNAL OF FLOW CHEMISTRY
卷 12, 期 2, 页码 227-235出版社
SPRINGER
DOI: 10.1007/s41981-022-00219-z
关键词
Kinetic resolution; Lipase; Micro-reactor; Hydrolysis
资金
- National Key Research and Development Program of China [2021YFC2101900]
- National Natural Science Foundation of China [22078150]
A strategy integrating biotechnology and microtechnology was developed to enhance the optical purity of mandelic acids during kinetic resolution process. The study showed that the method achieved higher optical purity under optimal conditions and improved the kinetics properties.
During the kinetic resolution process, increased substrate conversion often compromised the optical purity of products. A strategy integrated biotechnology and microtechnology was developed to kinetic resolution of mandelic acids. The effects of temperature, pH, solvent, reaction time, flow rate on the hydrolysis were conducted. Under optimal conditions, the MCR reaction afforded 95% ee(p), whereas the batch reaction yielded 65% ee(p). The kinetics of hydrolysis was also investigated. The value for K-m and V-max in batch was 12 mM and 11 mM s(-1), while that in MCR was 8 mM, 45 mM s(-1). This means more substrates can be transferred to the active site on the enzyme in MCR at the same concentration. This is a benefit from the excellent mass transfer limitations in MCR. Furthermore, the apparent V-max was significantly increased, closer to the intrinsic rate of reaction. Finally, the strong mass transfer in MCR leads to an expanded rate difference between (R) enantiomers and (S) enantiomers, improving the ee(p) and E-values.
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