期刊
CELL REPORTS
卷 39, 期 3, 页码 -出版社
CELL PRESS
DOI: 10.1016/j.celrep.2022.110696
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资金
- German Research Foundation [SFB1089, SP1775, SCHO 820/4-7, INST1172 15, DI853/3-5, DI853/3-7, INST 217/785-1]
- BONFOR program of the University of Bonn Medical Center
- National Health and Medical Research Project Grant [1079160]
- Cancer Institute New South Wales
- Australian Cancer Research Foundation, Australia
- University Hospital Bonn [SFB1089]
- Children's Medical Research Institute Biomedical Proteomics Facility
The stability of synaptic function relies on the ability of synapses to adjust their strength according to neuronal activity levels. This study reveals that the phosphorylation status of the active-zone protein RIM1 is crucial for synaptic glutamate release. Furthermore, the protein kinase SRPK2 and RIM1 are shown to play a role in the homeostatic balance of synaptic coupling in neuronal networks.
Stable function of networks requires that synapses adapt their strength to levels of neuronal activity, and failure to do so results in cognitive disorders. How such homeostatic regulation may be implemented in mammalian synapses remains poorly understood. Here we show that the phosphorylation status of several positions of the active-zone (AZ) protein RIM1 are relevant for synaptic glutamate release. Position RIMS1045 is necessary and sufficient for expression of silencing-induced homeostatic plasticity and is kept phosphorylated by serine arginine protein kinase 2 (SRPK2). SRPK2-induced upscaling of synaptic release leads to additional RIM1 nanoclusters and docked vesicles at the AZ and is not observed in the absence of RIM1 and occluded by RIMS1045E. Our data suggest that SRPK2 and RIM1 represent a presynaptic phosphosignaling hub that is involved in the homeostatic balance of synaptic coupling of neuronal networks.
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