期刊
CELL REPORTS
卷 39, 期 7, 页码 -出版社
CELL PRESS
DOI: 10.1016/j.celrep.2022.110830
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资金
- Wellcome Trust [215513, 203817]
X chromosome inactivation is mediated by Xist and involves chromatin modification and gene silencing. The RNA binding protein SPEN plays a central role in Xist-mediated gene silencing and also contributes to the correct localization of Xist RNA. SPEN and the Polycomb pathway function in parallel to establish gene silencing, while differentiation-dependent recruitment of SmcHD1 is necessary for silencing X-linked genes.
X chromosome inactivation (XCI) is mediated by the non-coding RNA Xist, which directs chromatin modification and gene silencing in cis. The RNA binding protein SPEN and associated corepressors have a central role in Xist-mediated gene silencing. Other silencing factors, notably the Polycomb system, have been reported to function downstream of SPEN. In recent work, we found that SPEN has an additional role in correct localization of Xist RNA in cis, indicating that its contribution to chromatin-mediated gene silencing needs to be reappraised. Making use of a SPEN separation-of-function mutation, we show that SPEN and Polycomb pathways, in fact, function in parallel to establish gene silencing. We also find that differentiation-dependent recruitment of the chromosomal protein SmcHD1 is required for silencing many X-linked genes. Our results provide important insights into the mechanism of X inactivation and the coordination of chromatin-based gene regulation with cellular differentiation and development.
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