期刊
ACS MACRO LETTERS
卷 11, 期 5, 页码 636-642出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsmacrolett.2c00095
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资金
- National Natural Science Foundation of China (NSFC) [51903202, 81903268]
- Key R&D Project in Agriculture and Animal Husbandrys of Rongchang, Chongqing [cstc2020ngzx0001]
- Fundamental Research Funds for the Central Universities [xtr042019020]
- Key R&D Program of Shaanxi Province [2020GXLH-Y016]
- Natural Science Foundation of Shaanxi Province [2020JM-055]
- Young Talents Support Plan of Xi'an Jiaotong University [HG6J002]
Biodegradable and lipid-like highly branched poly(beta-amino ester)s (HPAESA) were developed to enhance the biological functions of adipose-derived stem cells by gene transfection. HPAESA showed significantly higher efficiency and cell viability, as well as the ability to promote neurite outgrowth.
Biodegradable and lipid-like highly branched poly(beta-amino ester)s, HPAESA, were developed to enhance the biological functions of adipose-derived stem cells by gene transfection. Biodegradability reduces the cytotoxicity of HPAESA and enables controlled DNA release. Lipid mimicry enhances cellular uptake and endosomal escape of HPAESA/DNA polyplexes. HPAESA are able to transfect rat adipose-derived stem cells (rADSs) and human ADSCs (hADSCs) with orders of magnitude higher efficiency than commercial gene transfection reagents, with cell viability exceeding 90%. Most importantly, HPAESA can effectively transfer the nerve growth factor (NGF)-encoding plasmid to rADSCs and induce high NGF secretion, which significantly promotes neurite outgrowth of PC12 cells.
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