Tyrosine O-sulfation proteoforms affect HIV-1 monoclonal antibody potency

CAP256V2LS, a broadly neutralizing monoclonal antibody (bNAb), is being pursued as a promising drug for HIV-1 prevention. The total level of tyrosine-O-sulfation, a post-translational modification, was known to play a key role for antibody biological activity. More importantly, here wedescribe for the first time the significance of the tyrosine-O-sulfation proteoforms. We developed a hydrophobic interaction chromatography (HIC) method to separate and quantify different sulfation proteoforms, which led to the direct functionality assessment of tyrosine-sulfated species. The fully sulfated (4-SO3) proteoform demonstrated the highest in vitro relative antigen binding potency and neutralization efficiency against a panel of HIV-1 viruses. Interestingly, highly variable levels of 4-SO3 were produced by different clonal CHO cell lines, which helped the bNAb process development towards production of a highly potent CAP256V2LS clinical product with high 4-SO3 proteoform. This study presents powerful insight for any biotherapeutic protein development where sulfation may play an important role in product efficacy.

Automated summary

This study explores the potential of CAP256V2LS as a drug for HIV-1 prevention. The researchers developed a method to separate and quantify different sulfation proteoforms and found that the fully sulfated proteoform demonstrated the highest antigen binding and neutralization efficiency. The study also highlighted the variable levels of sulfation produced by different cell lines, which can aid in the development of more potent clinical products.