期刊
SCIENTIFIC REPORTS
卷 12, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41598-022-09886-3
关键词
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资金
- Westmead Institute of Medical Research
- Westmead Research Hub
This study aims to investigate the presence of tissue-resident memory T cells (T-RMs) in deeper layers of the healthy human conjunctiva. Using immunofluorescence microscopy, the researchers found CD69(+)T(RM) subsets in all layers of the conjunctiva, with higher densities in the adenoid layer. Interestingly, CD4 T-RMs were more abundant than CD8 T-RMs in the adenoid layer. These findings suggest the presence of defense mechanisms capable of inducing long-term immunogenic memory in the deep conjunctiva.
Mucosal linings of the body, including the conjunctiva, are enriched in tissue-resident memory T cells (T-RMs) whose defining feature is their continual tissue protection that does not rely on migration to lymphoid organs to elicit immune responses. Hitherto, conjunctival T-RMs have only been identified in the superficial epithelium. This work aims to develop a more complete understanding of the conjunctival immunological capacity by investigating the presence of T-RMs within the deeper, more stable layers of the healthy human conjunctiva. Using immunofluorescence microscopy and antibodies against CD3, CD4, CD69 and HLA-DR on bulbar conjunctival biopsies obtained from 7 healthy adults (age range = 32-77 years; females = 4), we identified CD69(+)T(RM) subsets in all layers of the human conjunctiva: the superficial epithelium, the basal epithelium, the adenoid, and the fibrous layers. Interestingly, the adenoid layer showed significantly higher densities of both CD4 and CD8 T-RMs when compared to the fibrous layer and conjunctival epithelia. Additionally, CD4 T-RMs predominated significantly over CD8 T-RMs in the adenoid layer. The abundance of deep conjunctival CD69(+)T(RMs) within the healthy human may suggest the presence of defence mechanisms capable of inducing long-term immunogenic memory. Understanding this spatial distribution of conjunctival CD69(+)T(RMs) is essential to improving mucosal vaccine design.
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