期刊
SCIENTIFIC REPORTS
卷 12, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41598-022-12571-0
关键词
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资金
- European Union [713694]
- European Research Council Synergy grant ASTRA [855923]
- Project LOCALSCENT [PROT. A0375-2020-36549]
Blind-structured illumination microscopy (blind-SIM) is enhanced by a custom convolutional neural network architecture (BS-CNN) which outperforms other blind-SIM deconvolution algorithms. It provides a resolution improvement of 2.17 and reduces artifacts. Additionally, BS-CNN is robust in cross-database variability.
Blind-structured illumination microscopy (blind-SIM) enhances the optical resolution without the requirement of nonlinear effects or pre-defined illumination patterns. It is thus advantageous in experimental conditions where toxicity or biological fluctuations are an issue. In this work, we introduce a custom convolutional neural network architecture for blind-SIM: BS-CNN. We show that BS-CNN outperforms other blind-SIM deconvolution algorithms providing a resolution improvement of 2.17 together with a very high Fidelity (artifacts reduction). Furthermore, BS-CNN proves to be robust in cross-database variability: it is trained on synthetically augmented open-source data and evaluated on experiments. This approach paves the way to the employment of CNN-based deconvolution in all scenarios in which a statistical model for the illumination is available while the specific realizations are unknown or noisy.
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