期刊
SCIENTIFIC REPORTS
卷 12, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41598-022-10612-2
关键词
-
资金
- UGA Franklin/OVPR seed grant program, UGA College of Agricultural and Environmental Sciences [NSF DBI-1946937]
- NSF [MCB-2041546]
- NSF Systems and Synthetic Biology (SSB)
- NSF Physics of Living Systems (PoLS)
We investigated the phase synchronization of cellular clocks and found that the macroscopic limit is 150,000 cells or less. The maximum radius of a hypothesized quorum sensing signal molecule was estimated to be 13.05 nm using a diffusion approximation for signal travel. By using a second microwell device, we refined the macroscopic limit to a cell density of 2166 cells per fixed area for phase synchronization.
We determined the macroscopic limit for phase synchronization of cellular clocks in an artificial tissue created by a big chamber microfluidic device to be about 150,000 cells or less. The dimensions of the microfluidic chamber allowed us to calculate an upper limit on the radius of a hypothesized quorum sensing signal molecule of 13.05 nm using a diffusion approximation for signal travel within the device. The use of a second microwell microfluidic device allowed the refinement of the macroscopic limit to a cell density of 2166 cells per fixed area of the device for phase synchronization. The measurement of averages over single cell trajectories in the microwell device supported a deterministic quorum sensing model identified by ensemble methods for clock phase synchronization. A strong inference framework was used to test the communication mechanism in phase synchronization of quorum sensing versus cell-to-cell contact, suggesting support for quorum sensing. Further evidence came from showing phase synchronization was density-dependent.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据