Micro-computed tomography assessment of bone structure in aging mice

High-resolution computed tomography (CT) is widely used to assess bone structure under physiological and pathological conditions. Although the analytic protocols and parameters for micro-CT (mu CT) analyses in mice are standardized for long bones, vertebrae, and the palms in aging mice, they have not yet been established for craniofacial bones. In this study, we conducted a morphometric assessment of craniofacial bones, in comparison with long bones, in aging mice. Although age-related changes were observed in the microarchitecture of the femur, tibia, vertebra, and basisphenoid bone, and were more pronounced in females than in males, the microarchitecture of both the interparietal bone and body of the mandible, which develop by intramembranous ossification, was less affected by age and sex. By contrast, the condyle of the mandible was more affected by aging in males compared to females. Taken together, our results indicate that mouse craniofacial bones are uniquely affected by age and sex.

Automated summary

In this study, a morphometric assessment of craniofacial bones was conducted in aging mice, and it was found that the microarchitecture of craniofacial bones is uniquely affected by age and sex. The interparietal bone and body of the mandible were less affected by age and sex, while the condyle of the mandible showed more pronounced changes in males compared to females.