期刊
SCIENTIFIC REPORTS
卷 12, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41598-022-09798-2
关键词
-
资金
- European Union [824110EASI-Genomics]
- Swedish Research Council [2019-01976]
- Goran Gustafsson Foundation
- SciLifeLab Uppsala Technology Development Grant
- Swedish Research Council
DNA methylation is an important epigenetic mark that can be detected in single cells using bisulfite sequencing. Various strategies for single-cell DNA methylation studies have been described, but limitations in cost-efficiency and yield remain. A new method, scSPLAT, demonstrates higher scale and throughput in sample preparation compared to other scWGBS methods.
DNA methylation is a central epigenetic mark that has diverse roles in gene regulation, development, and maintenance of genome integrity. 5 methyl cytosine (5mC) can be interrogated at base resolution in single cells by using bisulfite sequencing (scWGBS). Several different scWGBS strategies have been described in recent years to study DNA methylation in single cells. However, there remain limitations with respect to cost-efficiency and yield. Herein, we present a new development in the field of scWGBS library preparation; single cell Splinted Ligation Adapter Tagging (scSPLAT). scSPLAT employs a pooling strategy to facilitate sample preparation at a higher scale and throughput than previously possible. We demonstrate the accuracy and robustness of the method by generating data from 225 single K562 cells and from 309 single liver nuclei and compare scSPLAT against other scWGBS methods.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据