期刊
NUTRIENTS
卷 14, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/nu14051053
关键词
inflammatory bowel disease; microbiome; fibre; short-chain fatty acids; fermentation
资金
- Cure Crohn's Colitis
- The Catherine McEwan Foundation
- Biotechnology and Biological Sciences Research Council [BB/R006539/1]
Despite microbial dysbiosis, patients with IBD have a similar capacity to ferment fiber and release SCFA as healthy controls. Fiber supplementation alone may be unlikely to restore the compositional shifts characteristic of the IBD microbiome back to a healthy state.
Introduction: It has been suggested that the gut microbiome of patients with inflammatory bowel disease (IBD) is unable to ferment dietary fibre. This project explored the in vitro effect of fibre fermentation on production of short-chain fatty acids (SCFA) and on microbiome composition. Methods: Faecal samples were collected from 40 adults (>16 y) with IBD (n = 20 with Crohn's disease and n = 20 with ulcerative colitis) in clinical remission and 20 healthy controls (HC). In vitro batch culture fermentations were carried out using as substrates maize starch, apple pectin, raftilose, wheat bran, alpha cellulose and a mixture of these five fibres. SCFA concentration (umol/g) was quantified with gas chromatography and microbiome was profiled with 16S rRNA sequencing. Results: Fibre fermentation did not correct the baseline microbial dysbiosis or lower diversity seen in either patients with CD or UC. For all fibres, up to 51% of baseline ASVs or genera changed in abundance in HC. In patients with IBD, fermentation of fibre substrates had no effect on species or genera abundance. Production of SCFA varied among the different fibre substrates but this was not different between the two IBD groups and compared to HC after either 5 or 24 h fermentation. Conclusions: Despite extensive microbial dysbiosis, patients with IBD have a similar capacity to ferment fibre and release SCFA as HC. Fibre supplementation alone may be unlikely to restore to a healthy status the compositional shifts characteristic of the IBD microbiome.
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