期刊
GUT PATHOGENS
卷 14, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13099-022-00489-x
关键词
Enterotoxigenic Bacteroides fragilis; Toxin; Interleukin-8; IL-8; Stat3; E-cadherin; Colorectal cancer; Inflammation; HT29; HCT116; beta-catenin
资金
- Neil and Pearl Hamilton Trust
- Canterbury Medical Research Foundation (New Zealand)
ETBF infection activates IL-8 gene and protein expression through Stat3, while the disruption of E-cadherin appears to be independent of Stat3 signaling.
Background: Enterotoxigenic Bacteroides fragilis (ETBF) has been implicated in colorectal carcinogenesis through the actions of its toxin, B. fragilis toxin (BFT). Studies on colorectal cell lines have shown that treatment with BFT causes disruption of E-cadherin leading to increased expression of the pro-inflammatory cytokine, IL-8. Stat3 activation has also been associated with ETBF-related colitis and tumour development. However, a link between E-cadherin, IL-8 and Stat3 has not been investigated in the context of ETBF infection. Results: We found that co-culture of HT-29 and HCT116 colorectal cell lines with ETBF, had a similar effect on activation of IL8 gene and protein expression as treatment with purified BFT. Inhibition of Stat3 resulted in a decrease in IL-8 gene and protein expression in response to ETBF in both cell lines. A reduction in E-cadherin expression in response to ETBF treatment was not restored by blocking Stat3. Conclusion: We found that treatment of colorectal cancer cell lines with live cultures of ETBF had the equivalent effect on IL-8 expression as the use of purified toxin, and this may be a more representative model of ETBF-mediated colorectal carcinogenesis. IL-8 gene and protein expression was mediated through Stat3 in HT-29 and HCT116 cells, whereas disruption of E-cadherin appeared to be independent of Stat3 signalling.
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