期刊
CANCER DISCOVERY
卷 12, 期 5, 页码 1294-1313出版社
AMER ASSOC CANCER RESEARCH
DOI: 10.1158/2159-8290.CD-21-1207
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资金
- Total Cancer Care Research Protocol at the H. Lee Moffitt Cancer Center
- Moffitt Clinical Science Fund
- Miles for Moffitt fund
- Department of Cutaneous Oncology research support fund
- Cancer Center Support Grant (CCSG) [P30-CA076292]
- Donald A. Adam Melanoma and Skin Cancer Center of Excellence
- NIH [R01CA240434]
This study comprehensively investigated the tumor ecosystem of transformed CTCL using multiomics approaches. The findings revealed high tumor mutation burden, recurrently mutated pathways, and immune escape related changes in tCTCL. In addition, pharmacologic perturbation targeting OXPHOS and MYC showed promising antitumor activities, and intercellular communications between malignant T cells and macrophages/B cells were identified.
Cutaneous T-cell lymphoma (CTCL) is a rare cancer of skin-homing T cells. A subgroup of patients develops large cell transformation with rapid progression to an aggressive lymphoma. Here, we investigated the transformed CTCL (tCTCL) tumor ecosystem using integrative multiomics spanning whole-exome sequencing (WES), single-cell RNA sequencing, and immune profi ling in a unique cohort of 56 patients. WES of 70 skin biopsies showed high tumor mutation burden, UV signatures that are prognostic for survival, exome-based driver events, and most recurrently mutated pathways in tCTCL. Single-cell profi ling of 16 tCTCL skin biopsies identifi ed a core oncogenic program with metabolic reprogramming toward oxidative phosphorylation (OXPHOS), cellular plasticity, upregulation of MYC and E2F activities, and downregulation of MHC I suggestive of immune escape. Pharmacologic perturbation using OXPHOS and MYC inhibitors demonstrated potent antitumor activities, whereas immune profi ling provided in situ evidence of intercellular communications between malignant T cells expressing macrophage migration inhibitory factor and macrophages and B cells expressing CD74. SIGNIFICANCE: Our study contributes a key resource to the community with the largest collection of tCTCL biopsies that are diffi cult to obtain. The multiomics data herein provide the first comprehensive compendium of genomic alterations in tCTCL and identify potential prognostic signatures and novel therapeutic targets for an incurable T-cell lymphoma.
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