期刊
BMC PREGNANCY AND CHILDBIRTH
卷 22, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12884-022-04623-0
关键词
Preeclampsia; Oxidative stress; Free fatty acids; Trophoblast cells; Mitochondrial
资金
- Technology Project of Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University [YCXM19-23]
- Fujian Provincial Health Technology Project [2019-1-15]
- Startup Fund for scientific research, Fujian Medical University [2019QH1140]
- Joint Funds for the innovation of science and Technology, Fujian province [2020Y9163, 2020Y9134]
This study aimed to investigate the effects of free fatty acids on mitochondrial oxidative stress and the pathogenesis of preeclampsia. The results showed that serum free fatty acids might play an important role in the pathogenesis of preeclampsia by enhancing mitochondrial oxidative stress damage.
Aim To investigate the effects of free fatty acids on mitochondrial oxidative stress and the pathogenesis of preeclampsia. Methods Human primary trophoblast cells at 6-8 weeks of gestation were retrieved and cultured to 70-80% confluence, then incubated in serum from women with a normal pregnancy (normal pregnancy group), women with preeclampsia (PE group), and a combination of serum from women with 24 h preeclampsia-like symptoms and free fatty acids (FFA group). Mitochondrial membrane potential was assessed by fluorescent dye concurrent with detection of membrane channel conversion pore activity by fluorescence microscope. Enzyme labeling instruments and RT-PCR were used to detect mitochondrial DNA (mtDNA) levels. Results The preeclampsia and free fatty acids groups both exhibited significantly higher levels of mitochondria oxidative stress damage when compared to the normal pregnancy group. However, no significant differences in mitochondrial oxidative stress damage were observed between the FFA and PE groups. Conclusions Serum free fatty acids might play an important role in the pathogenesis of preeclampsia by enhancing mitochondrial oxidative stress damage.
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