4.8 Article

Are Vanadium Intermediates Suitable Mimics in Non-Heme Iron Enzymes? An Electronic Structure Analysis

期刊

ACS CATALYSIS
卷 12, 期 9, 页码 5489-5501

出版社

AMER CHEMICAL SOC
DOI: 10.1021/acscatal.2c01143

关键词

non-heme iron; vanadium mimics; density functional theory; metal-oxos; C; H activation

资金

  1. National Science Foundation [CBET-1704266, CBET-1846426, ACI-1548562]
  2. Career Award at the Scientific Interface from the Burroughs Wellcome Fund
  3. AAAS Marion Milligan Mason Award
  4. Alfred P. Sloan Fellowship in Chemistry

向作者/读者索取更多资源

In this study, the electronic structure of vanadyl mimics and catalytic iron intermediates were compared using density functional theory and correlated wavefunction theory. The calculations revealed crucial structural and energetic differences between the two due to their different spin states. The study also found that there were differences in the energetics for the isomerization and reaction of metal-oxo intermediates between vanadyl and ferryl mimics in non-heme iron enzymes.
Vanadyl intermediates are frequently used as mimics for the fleeting Fe(IV)=O intermediate in non-heme iron enzymes that catalyze C-H activation. Using density functional theory and correlated wavefunction theory, we investigate the degree to which the electronic structure of vanadium mimics is comparable to that of catalytic iron intermediates. Our calculations reveal crucial structural and energetic differences between vanadyl and ferryl intermediates primarily due to the former having a low-spin ground state and the latter having a high-spin one. This difference in spin state leads to differences in energetics for accessing isomers that confer activity in non-heme hydroxylase and halogenase enzymes. While interconversion between monodentate and bidentate succinate isomers of the key metal-oxo/hydroxo intermediates is energetically favorable for Fe, it is strongly unfavorable in V mimics. Additionally, isomerization of a terminal metal-oxo between an axial and equatorial position is energetically unfavorable for Fe but favorable for V. Analyses of binding strengths of Fe and V intermediates to alpha-ketoglutarate and succinate co-substrates reveal that both co-substrates bind more strongly to V than to Fe. Given the increasingly frequent use by the biochemistry community of V-based intermediates as mimics, our work highlights the limitations of this approach in studies of non-heme iron enzymes.

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