期刊
NATURE COMMUNICATIONS
卷 13, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41467-022-30406-4
关键词
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资金
- NIH [R01NS108151, R01GM121994, R01DK099023]
- G. Harold & Leila Y. Mathers Foundation
- TESS Research Foundation
- Wellcome Trust [210121/Z/18/Z]
- BBSRC [BB/V007424/1]
- American Cancer Society Postdoctoral Fellowship [129844-PF-17-135-01-TBE]
- Department of Defense Horizon Award [W81XWH-16-10153]
- Wellcome Trust [210121/Z/18/Z] Funding Source: Wellcome Trust
- BBSRC [BB/V007424/1] Funding Source: UKRI
In this study, the authors demonstrate that the VcINDY protein couples sodium and substrate binding through a cooperative mechanism by conformational selection. The results provide strong evidence for the sodium-dependent formation of the substrate binding site.
The Na+-dependent dicarboxylate transporter from Vibrio cholerae (VcINDY) is a prototype for the divalent anion sodium symporter (DASS) family. While the utilization of an electrochemical Na+ gradient to power substrate transport is well established for VcINDY, the structural basis of this coupling between sodium and substrate binding is not currently understood. Here, using a combination of cryo-EM structure determination, succinate binding and site-directed cysteine alkylation assays, we demonstrate that the VcINDY protein couples sodium- and substrate-binding via a previously unseen cooperative mechanism by conformational selection. In the absence of sodium, substrate binding is abolished, with the succinate binding regions exhibiting increased flexibility, including HP(in)b, TM10b and the substrate clamshell motifs. Upon sodium binding, these regions become structurally ordered and create a proper binding site for the substrate. Taken together, these results provide strong evidence that VcINDY's conformational selection mechanism is a result of the sodium-dependent formation of the substrate binding site. DASS carboxylate transporters linked to metabolic diseases couple the Na+ gradient to nutrient uptake. Here, the authors unravel the structural basis by which Na+ binding coordinates substrate interactions, revealing a distinct coupling mechanism.
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