期刊
NATURE COMMUNICATIONS
卷 13, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41467-022-29166-y
关键词
-
资金
- FET-Open [Dynamic-863203]
- European Research Council ERC Consolidator Grant [SMARTIES-724473]
- NIH [1RF1NS113251-01]
- China Scholarship Council (CSC) [201906960055]
- Chan Zuckerberg Initiative [DTI-0000000139]
The authors demonstrate a method for non-invasive fluorescence imaging behind scattering layers that extends beyond the optical memory effect. By demixing speckle patterns emitted by a fluorescent object using matrix factorization and fingerprint-based reconstruction, they achieve imaging with a larger field of view. Experimental validation confirms the efficiency and robustness of the method across various fluorescent samples.
The authors demonstrate non-invasive fluorescence imaging behind scattering layers beyond the optical memory effect. They achieve this by demixing speckle patterns emitted by a fluorescent object under variable unknown random illumination, using matrix factorization and a fingerprint-based reconstruction. Non-invasive optical imaging techniques are essential diagnostic tools in many fields. Although various recent methods have been proposed to utilize and control light in multiple scattering media, non-invasive optical imaging through and inside scattering layers across a large field of view remains elusive due to the physical limits set by the optical memory effect, especially without wavefront shaping techniques. Here, we demonstrate an approach that enables non-invasive fluorescence imaging behind scattering layers with field-of-views extending well beyond the optical memory effect. The method consists in demixing the speckle patterns emitted by a fluorescent object under variable unknown random illumination, using matrix factorization and a novel fingerprint-based reconstruction. Experimental validation shows the efficiency and robustness of the method with various fluorescent samples, covering a field of view up to three times the optical memory effect range. Our non-invasive imaging technique is simple, neither requires a spatial light modulator nor a guide star, and can be generalized to a wide range of incoherent contrast mechanisms and illumination schemes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据