Preparation and characterization of Meta-bromo-thiolactone calcium alginate nanoparticles

Recently, the focus has been shifting toward Quorum sensing inhibitors which reduce Pseudomonas aeruginosa virulence factors, alleviating infections. In this work, me-ta-bromo-thiolactone (mBTL), a potent quorum and virulence inhibitor for the Pseudomonas aeruginosa strains, were formulated in cal-cium alginate nanoparticles (CANPs). Alginate is used as nutrients and as backbone virulence aspect for Pseudomonas and therefore was chosen. mBTL-loaded-CANPs were characterized for particle size, polydispersity index, zeta potential, morphology visualized by Transmission Electron Microscopy (TEM) and drug release profile. Chemical and physical analysis of formulated mBTL-loaded-CANPs were evaluated using Fourier transform infrared Spectroscopy (FTIR) and differential scanning calorimetry (DSC) and Physical stability of mBTL-loaded-CANPs assessed at various temperature 25 +/- 1 degrees C, 4 +/- 0.5 degrees C and-30 degrees +/- 1 degrees C over a period of 4 and 9 months. Synthesized CANPs showed nano-size particles rang-ing from 140 to 200 nm with spherical particles for plain CANPS and irregular shape for mBTL-loaded-CANPs with a sustainable release profile over 48hrs. FTIR showed stable structure of loaded-mBTL and DSC displayed no interaction between mBTL and polymer. State of released mBTL from CANPs kept at 25 degrees C, 4 degrees C and-30 degrees C over 4 and 9 months showed stable formula at room temperature which kept as a goal of nanoparticles storage. The findings of this study revealed successful preparation of mBTL-loaded-CANPs.(c) 2022 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Automated summary

This study successfully prepared calcium alginate nanoparticles (CANPs) loaded with mBTL, a quorum and virulence inhibitor for Pseudomonas aeruginosa. The synthesized CANPs showed stable nano-size particles and sustained drug release profile. The mBTL-loaded-CANPs displayed stable structure and no interaction between mBTL and polymer. The findings suggest the potential of mBTL-loaded-CANPs in alleviating infections caused by Pseudomonas aeruginosa.