4.7 Article

Antioxidation and anti-inflammatory actions of the extract of Nitraria Tangutorum Bobr. fruits reduce the severity of ulcerative colitis in a dextran sulphate sodium-induced mice model

期刊

JOURNAL OF FUNCTIONAL FOODS
卷 91, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.jff.2022.105005

关键词

Nitraria Tangutorum Bobr. fruits; Antioxidant activity; Anti-inflammatory activity; Phenolic compounds; UC; MAPK/NF-kappa B

资金

  1. National Natural Science Foundation of China [81960777, 81660717, 81760745]
  2. Yunnan Ten-thousand Talents Program
  3. Union Special Fund of Yunnan Provincial Science and Technology Department and Yunnan University of Chinese Medicine [2019FF002]

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In this study, the compounds in the extract of Nitraria Tangutorum Bobr. fruits were characterized, and their antioxidant activities and anti-inflammatory effects were evaluated. The extract exhibited strong antioxidant activity and protected against DSS-induced UC in mice by inhibiting the activation of the MAPK/NF-κB signaling pathway.
In the present study, we characterised 25 compounds in the extract of Nitraria Tangutorum Bobr. fruits by using the HPLC/MS/MS method. We determined the antioxidant activities of ethanol, petroleum ether, chloroform (CE), ethyl acetate, n-butanol, and water extracts of N. Tangutorum fruits. All extracts were found to exhibit the antioxidant activity and significantly enhance the activities of antioxidant enzymes in H2O2-induced HepG-2 cells. Furthermore, the anti-inflammatory effect and molecular mechanism of the CE of N. Tangutorum Bobr. fruits were evaluated using the dextran sulphate sodium (DSS)-induced ulcerative colitis (UC) mouse model. CE significantly restored the body weight reduction, colon shortening, DAI elevation, and histological score in DSSinduced UC mice (P < 0.01). In addition, CE could remarkably decrease the levels of MPO, IL-1 beta, TNF-alpha, and IL-6 and increase the levels of superoxide dismutase, catalase, glutathione, and IL-10 in the colon of DSS mice (P < 0.01). According to western blot analysis, CE inhibited the MAPK/NF-kappa B signalling pathway activation. The results indicated that CE exerts a protective effect on DSS-induced UC mice, which may be related to the MAPK/NF-kappa B signalling pathway activation. In conclusion, CE can be used as a therapeutic drug for UC.

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