Applications of Surface Plasmon Resonance and Biolayer Interferometry for Virus-Ligand Binding

Surface plasmon resonance and biolayer interferometry are two common real-time and label-free assays that quantify binding events by providing kinetic parameters. There is increased interest in using these techniques to characterize whole virus-ligand interactions, as the methods allow for more accurate characterization than that of a viral subunit-ligand interaction. This review aims to summarize and evaluate the uses of these technologies specifically in virus-ligand and virus-like particle-ligand binding cases to guide the field towards studies that apply these robust methods for whole virus-based studies.

Automated summary

This review summarizes and evaluates the applications of surface plasmon resonance and biolayer interferometry, two commonly used real-time and label-free assays, in studies on virus-ligand interactions and virus-like particle-ligand binding. By examining and assessing the use of these methods, this review guides the field towards applying these robust techniques in whole virus-based studies.