期刊
VIRUSES-BASEL
卷 14, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/v14030621
关键词
foot-and-mouth disease; FMDV; vaccine; suspension BHK-21
类别
资金
- Bill & Melinda Gates Foundation [OPP1154700]
- BBSRC [BBS/E/I/00007037, BBS/E/I/00007039]
- Bill and Melinda Gates Foundation [OPP1154700] Funding Source: Bill and Melinda Gates Foundation
Foot-and-mouth disease (FMD) is a widespread disease that threatens food security and has severe economic impacts. Vaccination is the most effective control strategy, but current vaccines may not fully protect against different subtypes of the virus. Developing new vaccine strains is challenging due to difficulty in adapting the virus to cell culture. In this study, researchers have developed recombinant cell lines that express a key receptor for FMDV, which improves virus yield and can be used for future vaccine production.
Foot-and-mouth disease (FMD) is endemic in large parts of sub-Saharan Africa, Asia and South America, where outbreaks in cloven-hooved livestock threaten food security and have severe economic impacts. Vaccination in endemic regions remains the most effective control strategy. Current FMD vaccines are produced from chemically inactivated foot-and-mouth disease virus (FMDV) grown in suspension cultures of baby hamster kidney 21 cells (BHK-21). Strain diversity means vaccines produced from one subtype may not fully protect against circulating disparate subtypes, necessitating the development of new vaccine strains that antigenically match. However, some viruses have proven difficult to adapt to cell culture, slowing the manufacturing process, reducing vaccine yield and limiting the availability of effective vaccines, as well as potentiating the selection of undesired antigenic changes. To circumvent the need to cell culture adapt FMDV, we have used a systematic approach to develop recombinant suspension BHK-21 that stably express the key FMDV receptor integrin alpha v beta 6. We show that alpha v beta 6 expression is retained at consistently high levels as a mixed cell population and as a clonal cell line. Following exposure to field strains of FMDV, these recombinant BHK-21 facilitated higher virus yields compared to both parental and control BHK-21, whilst demonstrating comparable growth kinetics. The presented data supports the application of these recombinant alpha v beta 6-expressing BHK-21 in future FMD vaccine production.
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