Temperature-Mediated Effects on Mayaro Virus Vector Competency of Florida Aedes aegypti Mosquito Vectors

Mayaro virus (MAYV) is an emerging mosquito-borne arbovirus and public health concern. We evaluated the influence of temperature on Aedes aegypti responses to MAYV oral infection and transmission at two constant temperatures (20 degrees C and 30 degrees C). Infection of mosquito tissues (bodies and legs) and salivary secretions with MAYV was determined at 3, 9, 15, 21, and 27 days post ingestion. At both temperatures, we observed a trend of increase in progression of MAYV infection and replication kinetics over time, followed by a decline during later periods. Peaks of MAYV infection, titer, and dissemination from the midgut were detected at 15 and 21 days post ingestion at 30 degrees C and 20 degrees C, respectively. Mosquitoes were able to transmit MAYV as early as day 3 at 30 degrees C, but MAYV was not detectable in salivary secretions until day 15 at 20 degrees C. Low rates of MAYV in salivary secretions collected from infected mosquitoes provided evidence supporting the notion that a substantial salivary gland barrier(s) in Florida Ae. aegypti can limit the risk of MAYV transmission. Our results provide insights into the effects of temperature and time on the progression of infection and replication of MAYV in Ae. aegypti vectors.

Automated summary

The influence of temperature and time on Mayaro virus (MAYV) infection and replication in Aedes aegypti mosquitoes was evaluated. Higher temperature facilitated the transmission of MAYV, and the salivary gland barrier in Florida Ae. aegypti limited the risk of transmission.