期刊
WATER RESEARCH
卷 216, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.watres.2022.118303
关键词
Bacteriophages; Biocolloids; Heteroaggregation; Water treatment; Design
资金
- Center for the Environ-mental Implications of Nanotechnology (CEINT) under NSF [EF-0830093]
- NSF Convergence RAISE (Research Advanced by Interdisciplinary Science and Engineering) [1931309]
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [1931309] Funding Source: National Science Foundation
The study investigated the design options for implementing bacteriophages as targeted bacterial inactivation agents in water treatment systems. It focused on heteroaggregation, where phages attach to non-host background particles. The study found that heteroaggregation between bacteriophages and kaolinite occurred rapidly, likely driven by extended DLVO forces. The findings suggest that when using bacteriophages as inactivation agents, they should be used after particle separation processes to reduce the likelihood of scavenging through attachment to particulate matter rather than the targeted bacteria.
The validity and usefulness of implementing bacteriophages into water treatment systems as agents of targeted bacterial inactivation is yet to be determined. While some concerns are still more purely biological in nature other concerns are still chiefly rooted in design feasibility. This work investigated bacteriophage heteroaggregation, a process whereby phages attach to non-host background particles, to explore different design options for water quality engineers, especially tuning mixing velocity. This was done by adapting batch/mixing assays, originally developed to study inert particle heteroaggregation, to characterize bacteriophage and kaolinite heteroaggregation using modified Smoluchowski parameters under different ionic strength conditions. This work found that regardless of the ionic strength or the tested phage to kaolinite ratios heteroaggregation occurred rapidly and was likely driven by extended DLVO forces. A model of bacteriophage-kaolinite heteroaggregation was generated and showed promising correspondence with observed laboratory data. This model, along with other findings, suggests that should bacteriophages be utilized as agents of host inactivation they ought to be used following particle separation processes to reduce the likelihood of phage scavenging through attachment to particulate matter rather than the targeted bacteria.
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