Molecular-level composition of dissolved organic matter in distinct trophic states in Chinese lakes: Implications for eutrophic lake management and the global carbon cycle

Dissolved organic matter (DOM) is an abundant and mobile part of the aquatic environment and plays important roles in aquatic biogeochemical cycles and the global carbon cycle. Recently, eutrophication has become an important environmental issue in global lakes, but how eutrophication drives changes in the molecular composition of DOM along trophic gradients remains poorly understood. We thus characterized 67 DOM isolates from 11 lakes along a trophic gradient in China by using a combined approach including absorption spectroscopy, excitation-emission matrix fluorescence and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS). Our results indicated that dissolved organic carbon and absorption coefficients at 350 nm increased with increasing trophic status index. The ultraviolet absorbance at 254 nm and fluorescence intensity of all fluorescent components were higher in eutrophic lakes than in oligotrophic lakes. DOM in high trophic state lakes tended to be dominated by higher molecular weight, unsaturation degree, greater abundance of S -containing compounds, and condensed or polycyclic aromatic compounds than oligotrophic lakes. Additionally, autochthonous DOM characterized by more aliphatic compounds increased with the increasing trophic state. We concluded that nutrient input along with allochthonous DOM favors the lake eutrophication and subsequently increases the release and accumulation of autochthonous DOM. Consequently, eutrophication modifies the structure of the organic matter into more complex materials with increased input of allochthonous DOM and increased release of autochthonous DOM, which could accelerate global carbon cycle processes. Our results here have potential to contribute significantly to future studies of DOM dynamics in eutrophic lakes.

Automated summary

Dissolved organic matter (DOM) plays a crucial role in aquatic biogeochemical cycles and the global carbon cycle. This study investigates the molecular composition of DOM along a trophic gradient in 11 lakes in China. The results show that DOM from eutrophic lakes is characterized by higher molecular weight, unsaturation degree, abundance of S-containing compounds, and condensed or polycyclic aromatic compounds compared to DOM from oligotrophic lakes. In addition, nutrient input and allochthonous DOM contribute to lake eutrophication and the release of autochthonous DOM. This research provides valuable insights for future studies on DOM dynamics in eutrophic lakes.