Natural biofilm as a potential integrative sample for evaluating the contamination and impacts of PFAS on aquatic ecosystems

Natural biofilm can be a suitable medium for the monitoring of pollutants. Limited information is currently available regarding the occurrence of per- and polyfluoroalkyl substances (PFAS) in periphytic biofilm and low-trophic level organisms of freshwater ecosystems. In this study, surface water, biofilm, phytoplankton, and freshwater snails were collected from Taihu Lake, China, and characterized for 16 PFAS, including legacy compounds (PFSAs/PFCAs) and PFAS of emerging concern (fluorotelomer sulfonates and F-53B). The colonized biofilms effectively bioaccumulated PFAS from water, with the total concentration (sigma PFAS) in the range of 1.96-20.1 ng/g wet weight, and the bioaccumulation factor increased with the PFAS log K-ow values. As compared with phytoplankton, the sigma PFAS in biofilms displayed a stronger correlation with those in water. PFAS distinctly biomagnified from the biofilm to freshwater snail, with the biomagnification factor in the range of 3.09 & PLUSMN; 2.03 -17.8 & PLUSMN; 10.2, implying the important role of biofilm in PFAS transfer in aquatic environment. Extracellular proteins production in biofilm increased with the water PFAS concentrations. The total extracellular polymeric substances (EPS) content increased with the water PFAS concentration firstly and then declined to a steady level, while the algal chlorophyll level exhibited a similar relationship with the PFAS in biofilm. High PFAS levels were also associated with depressed alpha diversity of fungal community in biofilms. Biofilm appears as a relevant indicator to characterize the occurrence of PFAS in aquatic ecosystems.

Automated summary

Biofilms can serve as suitable media for monitoring pollutants, particularly per- and polyfluoroalkyl substances (PFAS). This study conducted research on PFAS in biofilms, phytoplankton, and freshwater snails collected from Taihu Lake, China. The results showed that biofilms effectively bioaccumulated PFAS from water, and PFAS distinctly biomagnified from the biofilm to freshwater snails. The production of extracellular proteins in biofilms increased with water PFAS concentrations.