4.5 Article

Comparison of immune response to human rhinovirus C and respiratory syncytial virus in highly differentiated human airway epithelial cells

期刊

VIROLOGY JOURNAL
卷 19, 期 1, 页码 -

出版社

BMC
DOI: 10.1186/s12985-022-01805-2

关键词

Human rhinovirus C; Air-liquid interface; Human bronchial epithelial cell; Respiratory syncytial virus; Immune response; Cytokines

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资金

  1. National Natural Science Foundation of China [81672020]

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This study found that HRV-C can infect and propagate in HBE cells, and significantly increase the secretion of various cytokines. Compared with RSV, HRV-C induces weaker cytokine expression.
Background Human rhinovirus C (HRV-C) accounts for a large proportion of HRV-related illnesses, but the immune response to HRV-C infection has not been elucidated. Our objective was to assess the effect of HRV-C on cytokine secretion in human bronchial epithelial (HBE) cells grown at air-liquid interface (ALI) and compare it with that of respiratory syncytial virus (RSV). Methods HBE cells were differentiated at ALI culture and the full-length cDNA clones of HRV-C651 and HRV-C15, clinical isolates of HRV-C79 and HRV-C101, and two RSV isolates were inoculated in the HBE cells. The effect of HRV-C on cytokine secretion was assessed and compared with that of RSV. Results HRV-Cs infect and propagate in fully differentiated HBE cells and significantly increase the secretion of IFN-lambda 1, CCL5, IP10, IL-6, IL-8, and MCP-1. The virus loads positively correlated with the levels of the cytokines. HRV-C induced lower secretion of CCL5 (P = 0.048), IL-6 (P = 0.016), MCP-1 (P = 0.008), and IL-8 (P = 0.032), and similar secretion of IP10 (P = 0.214) and IFN-lambda 1 (P = 0.214) when compared with RSV. Conclusion HBE ALI culture system supported HRV-C infection and propagation and HRV-C induced relatively weaker cytokine expression than RSV.

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