期刊
VETERINARY MICROBIOLOGY
卷 271, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.vetmic.2022.109477
关键词
TMUV; CTL epitope; ELISpot; Cytotoxic activity
资金
- National Key Research and Development Program of China [2016YFD0500107]
- Zhejiang Province Public Welfare Technology application Research Project [LGN22C010005]
This study predicted CTL epitope peptides of TMUV antigens and identified that 4 peptides could significantly induce cellular immune responses. Additionally, two H-2d-restricted CTL epitopes in BALB/c mice were identified, providing clues for further investigation of epitope vaccines against TMUV infection.
Since 2010, Tembusu virus (TMUV) has spread widely in China, causing huge economic losses to the poultry industry. Due to the infectious and zoonotic nature of flaviviruses, their potential threat to public health is of great concern. Cellular immune responses usually play a critical role in combating viral infections. To study the molecular basis of cell immunity induced by TMUV, 14 cytotoxic T lymphocyte (CTL) epitope peptides of TMUV antigen E, NS1 and NS3 were predicted by bioinformatics tools. Their abilities to induce cellular immune responses were determined by IFN-gamma ELISpot assay, and 4 peptides were found to exhibit highly significant responses upon stimulation. In addition, the cytotoxic activity induced by the epitope peptides was assessed by lactate dehydrogenase (LDH) release assay. Finally, among these peptides, we identified two murine TMUV NS3derived H-2d-restricted CTL epitopes in BALB/c mice, which could be used to further study of epitope vaccines against TMUV infection.
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