4.6 Review

Biomolecules capturing live bacteria from clinical samples

期刊

TRENDS IN BIOCHEMICAL SCIENCES
卷 47, 期 8, 页码 673-688

出版社

CELL PRESS
DOI: 10.1016/j.tibs.2022.03.018

关键词

-

资金

  1. Swiss National Science Foundation (SNSF) [407240_177368]
  2. Swiss National Science Foundation [310030_188817]
  3. SNSF Bridge grant [40B2-0_187170]
  4. European research Council (ERC) [772190]
  5. European Research Council (ERC) [772190] Funding Source: European Research Council (ERC)
  6. Swiss National Science Foundation (SNF) [407240_177368, 40B2-0_187170, 310030_188817] Funding Source: Swiss National Science Foundation (SNF)

向作者/读者索取更多资源

This article summarizes various biomolecules used for capturing live bacteria, including immune proteins, antibodies, aptamers, phage proteins, and antimicrobial peptides. The article also discusses the potential use of nanobodies targeting conserved surface-accessible proteins as promising biomolecules for pathogen capture.
Rapid phenotypic antimicrobial susceptibility testing (AST) requires the enrichment of live bacteria from patient samples, which is particularly challenging in the context of life-threatening bloodstream infections (BSIs) due to low bacterial titers. Over two decades, an extensive array of pathogen-specific biomolecules has been identified to capture live bacteria. The prevailing biomolecules are immune proteins of the complement system, antibodies, aptamers, phage proteins, and antimicrobial peptides. These biomolecules differ by their binder generation technologies and exhibit highly variable specificities, ranging from bacterial strains to most pathogenic bacteria. Here, we summarize how these diverse biomolecules were identified, list examples of successfully reported capture assays, and provide an outlook on the use of nanobodies raised against conserved surface-accessible proteins as promising biomolecules for pathogen capture.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据