4.4 Article

Melanocore uptake by keratinocytes occurs through phagocytosis and involves protease-activated receptor-2 internalization

期刊

TRAFFIC
卷 23, 期 6, 页码 331-345

出版社

WILEY
DOI: 10.1111/tra.12843

关键词

macropinocytosis; melanocore; melanosomes; phagocytosis; protease-activated receptor-2

资金

  1. European Commission [811087]
  2. Fundacao para a Ciencia e a Tecnologia [2020.8812, IF/00501/2014/CP1252/CT0001, PD/BD/114118/2015, PD/BD/137442/2018, PTDC/BIACEL/29765/2017]
  3. FCT/Ministerio da Ciencia, Tecnologia e Ensino Superior [UIDB/04462/2020, UIDP/04462/2020]
  4. Fundação para a Ciência e a Tecnologia [IF/00501/2014/CP1252/CT0001, PD/BD/137442/2018, PD/BD/114118/2015] Funding Source: FCT

向作者/读者索取更多资源

Different internalization pathways are followed by keratinocytes for the uptake of melanocores and melanosomes. Melanocores are internalized via phagocytosis, while melanosomes are internalized via macropinocytosis. Furthermore, the uptake of melanocores induces a higher level of protease-activated receptor-2 (PAR-2) internalization by keratinocytes compared to melanosomes, supporting the melanocore-based mechanism of melanin transfer.
In the skin epidermis, melanin is produced and stored within melanosomes in melanocytes, and then transferred to keratinocytes. Different models have been proposed to explain the melanin transfer mechanism, which differ essentially in how melanin is transferred-either in a membrane-bound melanosome or as a melanosome core, that is, melanocore. Here, we investigated the endocytic route followed by melanocores and melanosomes during internalization by keratinocytes, by comparing the uptake of melanocores isolated from the supernatant of melanocyte cultures, with melanosomes isolated from melanocytes. We show that inhibition of actin dynamics impairs the uptake of both melanocores and melanosomes. Moreover, depletion of critical proteins involved in actin-dependent uptake mechanisms, namely Rac1, CtBP1/BARS, Cdc42 or RhoA, together with inhibition of Rac1-dependent signaling pathways or macropinocytosis suggest that melanocores are internalized by phagocytosis, whereas melanosomes are internalized by macropinocytosis. Interestingly, we found that Rac1, Cdc42 and RhoA are differently activated by melanocore or melanosome stimulation, supporting the existence of two distinct routes of melanin internalization. Furthermore, we show that melanocore uptake induces protease-activated receptor-2 (PAR-2) internalization by keratinocytes to a higher extent than melanosomes. Because skin pigmentation was shown to be regulated by PAR-2 activation, our results further support the melanocore-based mechanism of melanin transfer and further refine this model, which can now be described as coupled melanocore exo/phagocytosis.

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