期刊
TOXICOLOGY AND APPLIED PHARMACOLOGY
卷 441, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.taap.2022.115971
关键词
Cytochalasin Q; Melanoma; Microfilament; Creatine kinase B; Creatine metabolism
资金
- National Natural Science Founda-tion of China [81872000, 82071749, 81373299]
- Hubei Provincial Key Research and Development Program [2020BHB019]
Targeting microfilaments with cytochalasins, such as cytochalasin Q (CQ), is considered a promising anticancer strategy. CQ was found to inhibit creatine metabolism and cytosolic glycolysis in melanoma cells by directly binding to and inactivating creatine kinase B (CKB). Furthermore, CQ and CKB depletion both suppressed the PI3K/AKT/FoxO1 pathway.
Due to the pivotal role of microfilament in cancer cells, targeting microfilaments with cytochalasins is considered a promising anticancer strategy. Here, we obtained cytochalasin Q (CQ) from Xylaria sp. DO1801, the endophytic fungi from the root of plant Damnacanthus officinarum, and discovered its anti-melanoma activity in vivo and in vitro attributing to microfilament depolymerization. Mechanistically, CQ directly bound to and inactivated creatine kinase B (CKB), an enzyme phosphorylating creatine to phosphocreatine (PCr) and regenerating ATP to cope with high energy demand, and then inhibited the creatine metabolism as well as cytosolic glycolysis in melanoma cells. Preloading PCr recovered ATP generation, reversed microfilament depolymerization and blunted anti-melanoma efficacy of CQ. Knockdown of CKB resulted in reduced ATP level, perturbed microfilament, inhibited proliferation and induced apoptosis, and manifested lower sensitivity to CQ. Further, we found that either CQ or CKB depletion suppressed the PI3K/AKT/FoxO1 pathway, whereas 740Y-P, a PI3K agonist, elevated protein expression of CKB suppressed by CQ. Taken together, our study highlights the significant anti melanoma effect and proposes a PI3K/AKT/FoxO1/ CKB feedback circuit for the activity of CQ, opening new opportunities for current chemotherapy.
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