期刊
TALANTA
卷 240, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.talanta.2021.123157
关键词
Lateral flow assay; Hook effect; Time conjugate release; Salivary assay; Fluorescence
资金
- RFF Forskningsfond Oslofjordfondet [285575]
- Regionalt forskningsfond Vestfold og Telemark [321814]
- Chongqing Research Program of Basic Research and Frontier Technology [cstc2019jcyj-msxmX0776, cstc2021jcyj-msxmX1038]
- Chongqing Education Commission - Science and Technology Research Program [KJZD-K201800802, KJZD-K201900802, KLZD-K202000805]
This paper presents a novel strategy of automatic timed detection antibody release to mitigate the high-dose hook effect in sandwich lateral flow assay (LFA) without additional manual steps. By introducing an intermediate pad treated with saturated sucrose solution, the flow between the nitrocellulose membrane and the conjugate pad is regulated to delay the reaction between detection antibodies and analytes. The strategy demonstrated a wider detection range without sacrificing the limit of detection compared to conventional LFA.
Sandwich lateral flow assay (LFA) is one of the most successfully commercialized paper-based biosensors, which offers a rapid, low-cost, one-step assay. Despite its advantages, conventional sandwich LFA is fundamentally limited by the high-dose hook effect-a phenomenon that occurs at very high analyte concentrations and results in false-negative results. In this paper, we present a novel strategy of automatic timed detection antibody release to mitigate the hook effect in sandwich LFA without additional manual steps. We introduced an intermediate pad treated with saturated sucrose solution to regulate the flow between the nitrocellulose membrane and the conjugate pad in order to delay the reaction between detection antibodies and analytes. Using C-reactive protein (CRP) as a representative analyte, we demonstrated that our strategy exhibited a range of detection 10 times wider than that of our conventional LFA, without sacrificing the limit of detection. Comparing to other published strategies, our work could offer a one-step, cost-effective approach that is closely unified with the benefits of the LFA.
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