期刊
SYSTEMATIC AND APPLIED MICROBIOLOGY
卷 45, 期 3, 页码 -出版社
ELSEVIER GMBH
DOI: 10.1016/j.syapm.2022.126316
关键词
Symbiovar; Beta-rhizobia; Symbiotic loci; nodA; nifH; Genealogical concordance
资金
- National Research Foundation (NRF)
- Department of Science and Innovation (DSI) of South Africa
- German Academic Exchange Service (DAAD)
- BBSRC [BB/L024209/1]
This study investigates the taxonomic status of rhizobial isolates from South African legume hosts. The isolates were initially identified as P. tuberum but were classified into two putative species clusters. Analysis of nucleotide identity confirmed the high genome similarity between isolates of one cluster and P. tuberum sensu stricto. A new taxon, P. podalyriae sp. nov., was proposed for the members of the other cluster associated with a single species of Podalyria.
Since the discovery of Paraburkholderia tuberum, an indigenous South African species and one of the first beta-rhizobia described, several other South African rhizobial Paraburkholderia species have been recognized. Here, we investigate the taxonomic status of 31 rhizobial isolates from the root nodules of diverse South African legume hosts in the Core Cape Subregion, which were initially identified as P. tuberum. These isolates originate from the root nodules of genera in the Papilionoideae as well as Vachellia karroo, from the subfamily Caesalpinioideae. Genealogical concordance analysis of five loci allowed delineation of the isolates into two putative species clusters (A and B). Cluster A included P. tuberum STM678T, suggesting that this monophyletic group represents P. tuberum sensu stricto. Cluster B grouped sister to P. tuberum and included isolates from the Paarl Rock Nature Reserve in the Western Cape Province. Average Nucleotide Identity (ANI) analysis further confirmed that isolates of Cluster A shared high genome similarity with P. tuberum STM678T compared to Cluster B and other Paraburkholderia species. The members of Cluster B associated with a single species of Podalyria, P. calyptrata. For this new taxon we accordingly propose the name Paraburkholderia podalyriae sp. nov., with the type strain WC7.3bT (= LMG 31413T; SARCC 750T). Based on our nodA and nifH phylogenies, P. podalyriae sp. nov. and strains of P. tuberum sensu stricto (including one from V. karroo) belong to symbiovar africana, the symbiotic loci of which have a separate evolutionary origin to those of Central and South American Paraburkholderia strains. (C) 2022 Elsevier GmbH. All rights reserved.
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