期刊
STRUCTURE
卷 30, 期 8, 页码 1088-+出版社
CELL PRESS
DOI: 10.1016/j.str.2022.05.008
关键词
-
资金
- JSPS/MEXT KAKENHI [JP21H05157, JP19K06526, JP22H02586, JP21H05155, JP21H05153, JP21K19226, JP21KK0125, JP19K22395, JP18KK0197, JP18H02405]
- Chemo-Sero-Therapeutic Research Institute
- Naito Foundation
- Takeda Science Foundation
- G-7 Scholarship Foundation
- Institute for Fermentation (Osaka)
- Yamada Science Foundation
- Deutsche Forschungsgemeinschaft (DFG
- German Research Foundation) [398967434 -TRR261]
- Juergen Manchot Foundation
- Platform Project for Supporting in Drug Discovery and Life Science Research (Platform for Drug Discovery, Informatics, and Structural Life Science) from Japan Agency for Medical Research and Development (AMED)
This study presents the crystal structures of inward-facing forms from Arsenophonus endosymbiont MurJ and an unprecedented squeezed form of Escherichia coli MurJ. Subsequent molecular dynamics simulations support the hypothesis that the squeezed form is an intermediate conformation.
The bacterial peptidoglycan enclosing the cytoplasmic membrane is a fundamental cellular architecture. The integral membrane protein MurJ plays an essential role in flipping the cell wall building block Lipid II across the cytoplasmic membrane for peptidoglycan biosynthesis. Previously reported crystal structures of MurJ have elucidated its V-shaped inward- or outward-facing forms with an internal cavity for substrate binding. MurJ transports Lipid II using its cavity through conformational transitions between these two forms. Here, we report two crystal structures of inward-facing forms from Arsenophonus endosymbiont MurJ and an unprecedented crystal structure of Escherichia coli MurJ in a squeezed'' form, which lacks a cavity to accommodate the substrate, mainly because of the increased proximity of transmembrane helices 2 and 8. Subsequent molecular dynamics simulations supported the hypothesis that the squeezed form is an intermediate conformation. This study fills a gap in our understanding of the Lipid II flipping mechanism.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据