Development of a new method based on gold nanoparticles for determination of uric acid in urine samples

In the current work, we have reported a fast and easy method based on gold nanoparticles for the deter-mination of uric acid in urine samples. In the first stage, gold nanoparticles were synthesized using the chemical reduction method and then applied as a sensor to measure uric acid concentration based on its strong reducing property. The main parameters affecting response signals such as pH, reagent concen-tration, and time are optimized using the multivariate method. Under the optimum conditions, the cal-ibration graphs were linear in the range of 0.5 - 10.0 mg.L-1 with limits of detection of 0.2 mg.L-1 and RSD% of 1.2% These results show that this nano-based method is a very sensitive and simple method for the determination of uric acid in urine samples. (C) 2022 Elsevier B.V. All rights reserved.

Automated summary

In this study, a fast and easy method based on gold nanoparticles was developed for the determination of uric acid in urine samples. The method utilized gold nanoparticles as a sensor to measure uric acid concentration based on its strong reducing property, and optimized the main parameters affecting response signals. The results demonstrated that this nano-based method is highly sensitive and simple for the determination of uric acid in urine samples.