An amplified fluorescence polarization assay for sensitive sensing of organophosphorus pesticides via MnO2 nanosheets

It is highly desirable to develop a simple, efficient and sensitive strategy for organophosphorus pesticides (OPs) in both environment pollution and human health. Herein, a novel amplified fluorescence polarization (FP) biosensor was established for highly sensitive detection of OPs using MnO2 nanosheets as the signal enhancer. In this system, OPs can suppress the activity of acetylcholinesterase (AChE) efficiently, blocking the hydrolysis reaction of acetylthiocholine (ATCh) to generate thiocholine (TCh) by AChE. TCh can lead the decomposition of MnO2 nanosheets to manganese ions. So, without the influence of TCh, MnO2 nanosheets can maintain its original shape and form a stable complex with FAM-DNA, which greatly enhanced the FP signal. This method can tremendously improve the sensitivity of FP with a detection limit of 0.01 ng/mL for diazinon. In addition, it was also applicable to determine other four OPs and investigate the level of diazinon in real water samples. Consequently, the proposed approach provides a new promising platform for detection of OPs and is expected to be used in application of environmental monitoring. (C) 2021 Published by Elsevier B.V.

Automated summary

A novel amplified fluorescence polarization biosensor was developed for highly sensitive detection of organophosphorus pesticides using MnO2 nanosheets as the signal enhancer. The method efficiently suppresses AChE activity and decomposes MnO2 nanosheets to enhance FP signal, tremendously improving the sensitivity of detection for OPs such as diazinon.