期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 273, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2022.121075
关键词
Hypochlorous acid; Ratiometric; ESIPT; ER-targeting; HepG2 cells
类别
资金
- Specialized Research Fund for the Doctoral Program of Higher Education [20123221110012]
A ratiometric fluorescent probe RHE was designed and synthesized for the detection of HOCl in the endoplasmic reticulum. The probe showed fast response, high sensitivity, and high selectivity, and it could accurately target the endoplasmic reticulum in cells.
Hypochlorous acid (HOCl), one of the most important ROS in living organisms, appears to serve an important role in the immune system in vivo. Endoplasmic reticulum (ER), the largest organelle in cells, manages many biological processes connected to vital activities. To better obtain insight into the relationship of ER stress and HOCl level, a ratiometric fluorescent probe RHE, based on rhodamine combined with HBT and ER-targeting group, was designed and synthesized for HOCl detection in the ER. Probe RHE shows a large stokes shift about 155 nm, which is derived to ESIPT principle. In addition, probe RHE exhibited excellent properties such as fast response (<80 s), high sensitivity with a low detection limit (40 nM), high selectivity and anti-interference. Moreover, probe RHE displayed an excellent ER-targeting ability and had been successfully applied for detection of exogenous and endogenous HOCl in HepG2 cells.(c) 2022 Elsevier B.V. All rights reserved.
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