期刊
SOFT MATERIALS
卷 20, 期 4, 页码 405-413出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/1539445X.2022.2053543
关键词
Gold-aryl nanoparticles; FRET; SARS-CoV-2 spike proteins; tryptophan
资金
- University of Sharjah [1602142-029-P, 150-2142-017-P]
Current research has focused on developing gold nanomaterials and methods for detecting the spike protein of SARS-CoV-2. The study found a strong binding affinity between the spike protein and tryptophan immobilized on gold-carbon nanoparticles, and used fluorescence quenching for detection.
Current research on coronavirus 2 disease (SARS-CoV-2) has been focused on developing reliable gold nanomaterials and methods for the detection of viral spike protein (S-proteins). Here, we describe the fluorescence quenching of spike proteins in the presence of robust gold-aryl nanoparticles. The obtained results demonstrated a strong binding affinity between S-proteins and tryptophan immobilized on gold-carbon nanoparticles (Trp-AuNPs) based on the binding constant value of 11.098 M-1. Forster non-radiative energy transfer (FRET) calculations showed an average binding distance of r = 3.06 nm and a critical energy transfer distance of R-0 = 2.5 nm. Time-resolved fluorescence studies of conjugated S-proteins onto Trp-AuNPs revealed a short fluorescence lifetime and dynamic quenching.
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